Number of the records: 1  

Comparison of specificity and sensitivity of immunochemical and molecular techniques for reliable detection of Erwinia amylovora

    1.
    SYSNO ASEP0095676
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JOstatní články
    TitleComparison of specificity and sensitivity of immunochemical and molecular techniques for reliable detection of Erwinia amylovora
    TitleSrovnání specificity a citlivosti imunochemických a molekulárních technik detekce Erwinia amylovora
    Author(s) Kokošková, B. (CZ)
    Mráz, Ivan (BC-A)
    Hýblová, Jana (UOCHB-X)
    Source TitleFolia Microbiologica. - : Springer - ISSN 0015-5632
    Roč. 52, č. 2 (2007), s. 175-182
    Number of pages8 s.
    Languageeng - English
    CountryCZ - Czech Republic
    KeywordsErwinia amylovora ; detection
    Subject RIVEE - Microbiology, Virology
    R&D Projects1QS500510558 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    CEZAV0Z50510513 - UMBR-M, BC-A (2005-2011)
    AnnotationErwinia amylovora, the causal agent of fire blight, was detected in plant samples and pure bacterial cultures by means of PCR, IFAS, and ELISA. Using the Biolog system and immature pear fruit assay, identities of all Ea strains were confirmed as the fire blight bacterium. In assays of pure Ea cultures, PTA-ELISA, and both IFAS and PCR were sensitive to concentrations 106-105 and 105-104 CFU/ml, respectively.
    WorkplaceBiology Centre (since 2006)
    ContactDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Year of Publishing2008
Number of the records: 1  

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