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Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering

SYSNO ASEP	0090142
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering
Title	Klonování genu kódujícího epoxidhydrolasu z Aspergillus niger M200, nadprodukce v E. coli a modifikace aktivity a enantioselectivity enzymu proteinovým inženýrstvím
Author(s)	Kotík, Michael (MBU-M)_{RID, ORCID} Štěpánek, Václav (MBU-M)_{RID, ORCID} Kyslík, Pavel (MBU-M)_{RID, ORCID} Marešová, Helena (MBU-M)_{RID, ORCID}
Source Title	Journal of Biotechnology. - : Elsevier - ISSN 0168-1656 Roč. 132, - (2007), s. 8-15
Number of pages	8 s.
Language	eng - English
Country	NL - Netherlands
Keywords	protein engineering ; saturation mutagenesis ; enantioselectivity
Subject RIV	EE - Microbiology, Virology
R&D Projects	GA204/06/0458 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
Annotation	The gene encoding an epoxide hydrolase from Aspergillus niger M200 has been clonek and its sequence determined. Saturation mutagenesis at codon 217 resulted in the discovery of nine enzyme variants showing in several cases profound differences in activity and enantioselectivity towards various epoxides when compared to the data of the wild-type enzyme. The site 217 is located at the entrance of the tunnel that provides the substrate with access to the active site
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2008

Number of the records: 1