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Functional activity of Gi alpha protein in detergent resistant membrane domains from rat brain cortex

SYSNO ASEP	0089344
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Functional activity of Gi alpha protein in detergent resistant membrane domains from rat brain cortex
Title	Funkční aktivita Gi alfa proteinu v detergent rezistentních membránových doménách mozkové kůry potkana
Author(s)	Stöhr, Jiří (FGU-C) Rudajev, Vladimír (FGU-C)_RID Bouřová, Lenka (FGU-C) Lisý, Václav (FGU-C) Novotný, Jiří (FGU-C) Svoboda, Petr (FGU-C)_{RID, ORCID}
Source Title	Journal of Neurochemistry. - : Wiley - ISSN 0022-3042 Roč. 101, Suppl.1 (2007), s. 52-52
Number of pages	1 s.
Action	European Society for Neurochemistry Meeting /17./
Event date	19.05.2007-22.05.2007
VEvent location	Salamanca
Country	ES - Spain
Event type	WRD
Language	eng - English
Country	US - United States
Keywords	cpo1 ; GABAB receptor ; Gi protein ; membrane domains
Subject RIV	ED - Physiology
CEZ	AV0Z50110509 - FGU-C (2005-2011)
Annotation	Instead of the prototypical detergent Triton X-100, we tried to find some other detergent(s) for membrane domains isolation, which would preserve GPCR/G protein coupling. Among various non-ionic detergents tested, Brij58 appeared to be the most convenient choice. We used baclofen-stimulated [35S]GTPγS binding study or measurement of high-affinity [γ32P]GTPase activity as valuable tools for assessment of direct functional coupling between the GABAB receptors and their cognate Gi proteins
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2008

Number of the records: 1