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Volume visualization of large biological tissue specimens captured by a confocal laser scanning microscope
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SYSNO ASEP 0087376 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Volume visualization of large biological tissue specimens captured by a confocal laser scanning microscope Title Objemová vizualizace obrazů velkých biologických tkáňových vzorků nasnímaných laserovým konfokálním mikroskopem Author(s) Čapek, Martin (FGU-C) RID, ORCID
Janáček, Jiří (FGU-C) RID, ORCID
Karen, Petr (FGU-C)
Kubínová, Lucie (FGU-C) RID, ORCID
Hána, P. (CZ)
Smrčka, P. (CZ)Source Title ICS´12. - Saint-Etienne : International society for stereology, 2007 Pages s. 12-17 Number of pages 6 s. Publication form flash-disc - flash-disc Action International congress for stereology /12./ Event date 03.09.2007-07.09.2007 VEvent location Saint-Etienne Country FR - France Event type WRD Language eng - English Country FR - France Keywords confocal microscope ; volume visualization Subject RIV JC - Computer Hardware ; Software R&D Projects LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA100110502 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA600110507 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA304/05/0153 GA ČR - Czech Science Foundation (CSF) IAA500200510 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50110509 - FGU-C (2005-2011) Annotation We apply volume reconstruction for visualization of biological specimens larger than the field of view of a confocal laser scanning microscope (CLSM). The first step of volume reconstruction is cutting large tissue specimens into thin physical slices. Second, volume image data sets (spatial tiles which overlap) are captured from all studied physical slices by CLSM. The third step is merging of overlapping spatial tiles of the same physical slice in horizontal direction (mosaicing). The fourth reconstruction step is merging of volumes of successive physical slices in vertical direction by applying an elastic registration algorithm. The last step is an image enhancement to compensate for the effect of the light attenuation with depth occurring in confocal microscopy. The resulting large digital volumes are visualized by a hardware accelerated volume rendering. In this paper we show a reconstruction of a middle part of a 21-days-old laboratory Norway rat embryo Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2008
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