Rapid subtyping of tick-borne encephalitis virus isolates using multiplex RT-PCR

Růžek, Daniel; Šťastná, B.; Kopecký, Jan; Golovljova, I.; Grubhoffer, Libor

Number of the records: 1

Rapid subtyping of tick-borne encephalitis virus isolates using multiplex RT-PCR

1.

SYSNO ASEP	0087201
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Rapid subtyping of tick-borne encephalitis virus isolates using multiplex RT-PCR
Title	Subtypová charakterizace izolátů viru klíšťové encefalitidy metodou multiplexové RT-PCR
Author(s)	Růžek, Daniel (BC-A)_{RID, ORCID} Šťastná, B. (CZ) Kopecký, Jan (BC-A)_RID Golovljova, I. (EE) Grubhoffer, Libor (BC-A)_{RID, ORCID}
Source Title	Journal of Virological Methods. - : Elsevier - ISSN 0166-0934 Roč. 144, 1/2 (2007), s. 133-137
Number of pages	5 s.
Language	eng - English
Country	NL - Netherlands
Keywords	tick-borne encephalitis virus ; subtyping ; multiplex RT-PCR
Subject RIV	EE - Microbiology, Virology
R&D Projects	LC06009 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA524/06/1479 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
Annotation	Tick-borne encephalitis virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of tick-borne encephalitis virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific signature positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of tick-borne encephalitis virus.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2008

Number of the records: 1