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Production, purification and oxidative folding of the mouse recombinant prion protein
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SYSNO ASEP 0087156 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Production, purification and oxidative folding of the mouse recombinant prion protein Title Produkce, purifikace a oxidatinvní folding myšího recombinantního prionového proteinu Author(s) Pavlíček, A. (CZ)
Bednárová, Lucie (UOCHB-X) RID, ORCID
Holada, K. (CZ)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 52, č. 4 (2007), s. 391-397Number of pages 7 s. Language eng - English Country CZ - Czech Republic Keywords recombinant prion protein ; production ; purification ; folding Subject RIV CE - Biochemistry R&D Projects GD310/05/H533 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40550506 - UOCHB-X (2005-2011) Annotation The method of overexpression of the full-length mouse recombinant prion protein in cytoplasm of E.coli and its effective purification is described. The folding state of the protein was studied using circular dichroism spectroscopy, by the resistance to cleavage by proteinase K and by centrifugation in suchrose gradient. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2008
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