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Effect of Protein Kinase Inhibitors on Protein Phosphorylation and Germination of Aerial Spores from Streptomyces coelicolor
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SYSNO ASEP 0084702 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Effect of Protein Kinase Inhibitors on Protein Phosphorylation and Germination of Aerial Spores from Streptomyces coelicolor Title Účinek inhibitorů protein kinas na fosforylaci proteinů a germinaci vzdušných spor Streptomyces coelicolor Author(s) Palečková, Petra (MBU-M)
Kontrová, K. (CZ)
Kofroňová, Olga (MBU-M) RID, ORCID
Bobek, Jan (MBU-M)
Benada, Oldřich (MBU-M) ORCID, RID
Mikulík, Karel (MBU-M)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 52, č. 3 (2007), s. 215-222Number of pages 8 s. Language eng - English Country CZ - Czech Republic Keywords streptomyces coelicolor ; protein kinase ; phosphoprotein Subject RIV EE - Microbiology, Virology R&D Projects GA203/05/0106 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) Annotation In vitro phosphorylation reaction using extracts prepared from cells of exponential phase of growth and aerial spores of Streptomyces coelicolor revealed presence of multiply phosphorylated proteins. We investigated effect of protein kinase inhibitors geldanamycin, wortmannin, apigenin, genistein, roscovitine, 2, 5-dihydroxycinnamic acid methyl ester, rapamycin and staurosporine on protein phosphorylation and on germination of spores. The in vitro experiments showed differences in phosphoprotein pattern due to the presence of protein kinase inhibitors. Cultivation of aerial spores with protein kinase inhibitors led to significant delay in germ tube emergence and filament formation. However, none of tested protein kinase inhibitors completely block the germination process. These results indicate protein kinases of spores form complex networks sharing common modulating site that plays an important role in proper timing of early developmental events Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2008
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