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Primary enamel knot cell death in Apaf-1 and caspase-9 deficient mice
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SYSNO ASEP 0082446 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Primary enamel knot cell death in Apaf-1 and caspase-9 deficient mice Title Buněčná smrt v primárním sklovinném uzlu u Apaf-1 a kaspáza-9 deficientních myší Author(s) Šetková, Jana (UZFG-Y)
Matalová, Eva (UZFG-Y) RID
Sharpe, P. T. (GB)
Míšek, Ivan (UZFG-Y)
Tucker, A. S. (GB)Source Title Archives of Oral Biology. - : Elsevier - ISSN 0003-9969
Roč. 52, 1 (2007), s. 15-19Number of pages 5 s. Language eng - English Country US - United States Keywords dental apoptosis ; apoptosome ; apaf-1 knockout ; caspase-9 knockout Subject RIV FF - HEENT, Dentistry R&D Projects KJB500450503 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA304/04/0101 GA ČR - Czech Science Foundation (CSF) OC B23.001 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50450515 - UZFG-Y (2005-2011) Annotation During molar development, apoptosis occurs in a well-characterized pattern suggesting several roles for cell death in odontogenesis. However, molecular mechanisms of dental apoptosis are only poorly understood. In this study, Apaf-1 and caspase-9 knockouts were used to uncover the engagement of these members of the apoptotic machinery during early tooth development, concentrating primarily on their function in the apoptotic elimination of primary enamel knot cells. Molar tooth germ morphology, proliferation and apoptosis were investigated on frontal histological sections of murine heads at embryonic days (ED) 15.5 - the stage when the primary enamel knot is eliminated apoptotically. In molar tooth germs of both knockouts, no apoptosis was observed according to morphological (haematoxylin-eosin) as well as biochemical criteria (TUNEL). Morphology of the mutant tooth germs, however, was not changed. Additionally, knockout mice showed no changes in proliferation compared to wild type mice. According to our findings on knockout embryos, Apaf-1 and caspase-9 are involved in apoptosis during tooth development; however, they seem dispensable and not necessary for proper tooth shaping. Compensatory or other mechanisms of cell death may act to eliminate the primary enamel knot cells in the absence of Apaf-1 and caspase-9. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2007
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