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Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis
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SYSNO ASEP 0082318 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis Title Zvýšená exprese farnesyl difosfát syntázy vyvolává na světle tvorbu lézí a pozměňuje homeostázi cytokininů Author(s) Manzano, D. (GB)
Busquets, A. (ES)
Closa, M. (ES)
Hoyerová, Klára (UEB-Q) RID, ORCID
Schaller, H. (FR)
Kamínek, Miroslav (UEB-Q) RID, ORCID
Arró, M. (ES)
Ferrer, A. (ES)Source Title Plant Molecular Biology. - : Springer - ISSN 0167-4412
Roč. 61, 1-2 (2006), s. 195-213Number of pages 19 s. Language eng - English Country NL - Netherlands Keywords Arabidopsis thaliana ; cytokinin ; farnesyl diphosphate synthase ; isoprenoid Subject RIV EF - Botanics R&D Projects IAA600380507 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50380511 - UEB-Q (2005-2011) Annotation To investigate the role of mitochondrial farnesyl diphosphate synthase (FPS) in plant isoprenoid biosynthesis we characterized transgenic Arabidopsis thaliana plants overexpressing FPS1L isoform. This overexpressed protein was properly targeted to mitochondria yielding a mature and active form of the enzyme of 40 kDa. Leaves from transgenic plants grown under continuous light exhibited symptoms of chlorosis and cell death correlating to H2O2 accumulation, and leaves detached from the same plants displayed accelerated senescence. Overexpression of FPS in mitochondria also led to altered leaf cytokinin profile, with a reduction in the contents of physiologically active trans-zeatin- and isopentenyladenine-type cytokinins and their corresponding riboside monophosphates as well as enhanced levels of cis-zeatin 7-glucoside and storage cytokinin O-glucosides. Overexpression of 3-hydroxy-3-methylglutaryl coenzyme A reductase did not prevent chlorosis in plants overexpressing FPS1L, but did rescue accelerated senescence of detached leaves and restored wild-type levels of cytokinins. We propose that the overexpression of FPS1L leads to an enhanced uptake and metabolism of mevalonic acid-derived isopentenyl diphosphate and/or dimethylallyl diphosphate by mitochondria, thereby altering cytokinin homeostasis and causing a mitochondrial dysfunction that renders plants more sensitive to the oxidative stress induced by continuous light. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2007
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