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Investigating oligonucleotide hybridization at subnanomolar level by surface plasmon resonance biosensor method

SYSNO ASEP	0040514
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Investigating oligonucleotide hybridization at subnanomolar level by surface plasmon resonance biosensor method
Title	Výzkum interakcí oligonukleotidů na subnanomolární úrovni pomocí senzoru s povrchovými plazmony
Author(s)	Vaisocherová, Hana (URE-Y)_RID Zítová, Alice (URE-Y) Lachmanová, Markéta (URE-Y) Štepánek, J. (CZ) Králíková, Šárka (UOCHB-X)_RID Liboska, Radek (UOCHB-X)_{RID, ORCID} Rejman, Dominik (UOCHB-X)_{RID, ORCID} Rosenberg, Ivan (UOCHB-X)_{RID, ORCID} Homola, Jiří (URE-Y)_RID
Source Title	Biopolymers - ISSN 0006-3525 Roč. 82, č. 4 (2006), s. 394-398
Number of pages	5 s.
Action	European Conference on the Spectroscopy of Biological Molecules - ECSBM 2005 /11./
Event date	03.09.2005-08.09.2005
VEvent location	Aschaffenburg
Country	DE - Germany
Event type	WRD
Language	eng - English
Country	US - United States
Keywords	surface plasmon resonance ; biosensors ; optical sensors
Subject RIV	BO - Biophysics
R&D Projects	GA303/03/0249 GA ČR - Czech Science Foundation (CSF)
	GA102/03/0633 GA ČR - Czech Science Foundation (CSF)
	GA202/05/0628 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z20670512 - URE-Y (2005-2011)
	AV0Z40550506 - UOCHB-X (2005-2011)
Annotation	We optimized surface plasmon resonance (SPR) biosensor for a rapid, direct, sensitive and label-free screening of synthetic oligonucleotides (ONs) with modified structure potentially applicable in antisense therapy. An optimized immobilization method was employed to obtain desired ligand density and hybridization efficiency. It was showed that the sensor is capable of detecting ONs in concentrations as low as 0.1 nM with high specificity and reproducibility.
Workplace	Institute of Radio Engineering and Electronics
Contact	Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488
Year of Publishing	2007

Number of the records: 1