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Pore-Forming and Enzymatic Activities of Bordetella pertussis Adenylate Cyclase Toxin Synergize in Promoting Lysis of Monocytes

SYSNO ASEP	0040415
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Pore-Forming and Enzymatic Activities of Bordetella pertussis Adenylate Cyclase Toxin Synergize in Promoting Lysis of Monocytes
Title	Kanálotvorná a enzymatická aktivita Adenylát cyklázového toxinu Bordetella pertussis synergizují v působení lyze monocytů
Author(s)	Basler, Marek (MBU-M) Mašín, Jiří (MBU-M)_{RID, ORCID} Osička, Radim (MBU-M)_{RID, ORCID} Šebo, Peter (MBU-M)_{RID, ORCID}
Source Title	Infection and Immunity. - : American Society for Microbiology - ISSN 0019-9567 Roč. 74, č. 5 (2006), s. 2207-2214
Number of pages	8 s.
Language	eng - English
Country	US - United States
Keywords	bordetella pertussis ; cyaa ; cytotoxicity
Subject RIV	EE - Microbiology, Virology
R&D Projects	IAA5020406 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
Annotation	We used here the non-acylated proCyaA, unable to form membrane pores, and the specific CyaA mutants exhibiting a selectively reduced hemolytic activity but full capacity to elevate cAMP in cells, to corroborate the observation that enzymatic depletion of cellular ATP due to toxin action is sufficient to promote cell death and lysis. It is further shown, that pore-forming (hemolytic) activity of CyaA synergizes with the invasive AC enzyme activity in maximizing the overall cytolytic potency of the toxin
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2007

Number of the records: 1