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Bordetella adenylate cyclase toxin induces a cascade of morphological changes of sheep erythrocytes and localizes into clusters in erythrocyte membranes

SYSNO ASEP	0040411
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Bordetella adenylate cyclase toxin induces a cascade of morphological changes of sheep erythrocytes and localizes into clusters in erythrocyte membranes
Title	Adenylátcyklázový toxin bakterie Bordetella indukuje kaskádu morfologických změn ovčích erytrocytů a nachází se ve shlucích v erytrocytární membráně
Author(s)	Vojtová, Jana (MBU-M)_{RID, ORCID} Kofroňová, Olga (MBU-M)_{RID, ORCID} Šebo, Peter (MBU-M)_{RID, ORCID} Benada, Oldřich (MBU-M)_{ORCID, RID}
Source Title	Microscopy Research and Technique - ISSN 1059-910X Roč. 69, - (2006), s. 119-129
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	cyaa ; scanning electron microscopy ; transmission electron microscopy
Subject RIV	EE - Microbiology, Virology
R&D Projects	IAA5020406 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
Annotation	This research article describes effects of Adenylate cyclase toxin (CyaA) of Bordetella pertussis on morphology of sheep erythrocytes and its localization in erythrocyte membranes. CyaA was shown to induce a cascade of morphological changes of erythrocytes, such as shrinkage, formation of membrane projections, and blebs and swelling. Further, immunolabeling of inserted CyaA-232/FLAG molecules with specific anti-FLAG antibodies and IgG-gold particles indicated a clustered distribution of CyaA molecules in erythrocyte membrane. This was confirmed by immunofluorescence and confocal microscopy, which revealed uniform stoichiometry of CyaA clusters, suggesting CyaA binding into specific domains in erythrocyte membrane
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2007

Number of the records: 1