Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae
1.
SYSNO ASEP
0040292
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae
Title
Kompartmentace rostlinného transporteru založená na lipidech
Author(s)
Grossmann, Q. (DE) Opekarová, Miroslava (MBU-M) Nováková, L. (DE) Stolz, J. (DE) Tanner, W. (DE)
Source Title
Eukaryotic Cell
- ISSN 1535-9778
Roč. 5, č. 6 (2006), s. 945-953
Number of pages
9 s.
Language
eng - English
Country
US - United States
Keywords
saccharomyces cerevisiae ; plant transport protein ; hup1
Subject RIV
EE - Microbiology, Virology
R&D Projects
LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ
AV0Z50200510 - MBU-M (2005-2011)
Annotation
The hexose-proton symporter HUP1 shows a spotty distribution in the plasma membrane of the green alga Chlorella kessleri. Chlorella cannot be transformed so far. To study the membrane localization of the HUP1 protein in detail, the symporter was fused to green fluorescent protein (GFP) and heterologously expressed in Saccharomyces cerevisiae and Schizosaccharomyces pombe. In these organisms, the HUP1 protein has previously been shown to be fully active. The GFP fusion protein was exclusively targeted to the plasma membranes of both types of fungal cells. In S. cerevisiae, it was distributed nonhomogenously and concentrated in spots resembling the patchy appearance observed previously for endogenous H(+) symporters. It is documented that the Chlorella protein colocalizes with yeast proteins that are concentrated in 300-nm raft-based membrane compartments
