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Cytokine production by porcine mononuclear leukocytes stimulated by mitogens

SYSNO ASEP	0030947
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Cytokine production by porcine mononuclear leukocytes stimulated by mitogens
Title	Produkce cytokinů mononukleárními leukocyty stimulovanými mitogeny u prasete domácího
Author(s)	Rašková, G. (CZ) Kovářů, František (UZFG-Y) Bártová, J. (CZ)
Source Title	Acta veterinaria Brno. - : Veterinární a farmaceutická univerzita Brno - ISSN 0001-7213 Roč. 74, - (2005), s. 521-525
Number of pages	5 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	cytokine ; ELISpot ; mitogen
Subject RIV	ED - Physiology
R&D Projects	GA524/05/0267 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
Annotation	The aim of the study was to contribute to the understanding of the postnatal development of the immune system in domestic pigs by studying in vitro cytokine production by mononuclear leukocytes stimulated with pokeweed mitogen and concanavalin A. The production of interleukin 1 beta, interferon gama, and tumour necrosis factor alfa was evaluated. The mitogenic effect was studied in cultures of two different cell concentrations and assessed by the ELISpot method, The stimulatory effect was detected in IL-1beta-secreting cells after exposure to PWM (stimulation index, 6.15 and 5.42)and to Con A (stimulation index, 2.12 and 2.63). IFN-gama-secreting cells at the concentration of 1x10 5 cells/ml responded to PWM to a low degree (stimulation index 1.9). Con A alone or combined with PWM had no stimulatory effect on either IFN-gama- or TNF-alfa-secreting cells, as compared with the controls.
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2006

Number of the records: 1