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Comparative phenotypic characterization of keratinocytes originating from hair follicles
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SYSNO ASEP 0029197 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Comparative phenotypic characterization of keratinocytes originating from hair follicles Title Srovnávací fenotypová charakterizace keratinocytů pocházejících z vlasových folikulů Author(s) Klíma, Jiří (UZFG-Y) RID, ORCID
Smetana Jr., K. (CZ)
Motlík, Jan (UZFG-Y) RID, ORCID
Plzáková, Z. (CZ)
Liu, F. T. (US)
Štork, J. (CZ)
Kaltner, H. (DE)
Chovanec, M. (CZ)
Dvořánková, B. (CZ)
André, S. (DE)
Gabius, H. J. (DE)Source Title Journal of Molecular Histology
Roč. 36, - (2005), s. 89-96Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords hair follicles Subject RIV EB - Genetics ; Molecular Biology R&D Projects LN00A065 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IBS4050005 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA304/04/0171 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50450515 - UZFG-Y (2005-2011) Annotation The principal pool of epidermal stem cells is located in the bulge region of the hair follicle root sheath. In this research project, we have used a refined procedure to isolate porcine hair follicles including their root sheath and for comparison purposes also human cell material. These cells migrating from the hair follicles were then cytochemically characterized. A panel of antibodies and two labeled plant lectins were tested on cell material obtained under a range of assorted experimental conditions. Due to their role in growth regulation we also studied two endogenous lectins, specifically monitoring their expression and the presence of accessible ligands. These in vitro results were compared with findings on porcine and human hair follicles and human basal cell carcinomas in situ. The keratinocytes originating from hair follicles in the presence of feeder cells are rather undifferentiated and express galectin-1/galectin-1-binding sites but not galectin-3 in their nuclei associated with DeltaNp63alpha positivity. Nuclear reactivity for galectin-1 was rarely observed in the bulge of the outer root sheath of the human hair follicle and of basal cell carcinomas and absent in porcine tissue samples. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2006
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