Requirement of the Caenorhabditis elegans RapGEF pxf-1 and rap-1 for epithelial integrity
1.
SYSNO ASEP
0025545
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Requirement of the Caenorhabditis elegans RapGEF pxf-1 and rap-1 for epithelial integrity
Title
RapGEF geny pxf-1 and rap-1 jsou u Caenorhabditis elegans nezbytné pro epiteliální integritu
Author(s)
Pellis-van Berkel, W. (NL) Verheijen, M. H. G. (NL) Cuppen, E. (NL) Asahina, Masako (PAU-O) de Rooij, J. (NL) Jansen, G. (NL) Plasterk, R. H. A. (NL) Bos, J. L. (NL) Zwartkruis, F. J. T. (NL)
Source Title
Molecular Biology of the Cell
- ISSN 1059-1524
Roč. 16, č. 1 (2005), s. 106-116
KJB5022303 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ
AV0Z60220518 - PAU-O, BC-A (2005-2011)
Annotation
The Rap-pathway has been implicated in various cellular processes but its exact physiological function remains poorly defined. We have shown that the C. elegans homologue of the mammalian guanine nucleotide exchange factors PDZ-GEFs, PXF-1, specifically activates Rap1 and Rap2. Deletion of the catalytic domain from pxf-1 leads to hypodermal defects, resulting in lethality. The cuticle secreted by pxf-1 mutants is disorganized and often not shed properly during molting. Disruption of rap-1 leads to a similar, but less severe phenotype, which is enhanced by the simultaneous removal of rap-2. The results demonstrate that the pxf-1/rap pathway in C. elegans is required for maintenance of epithelial integrity, in which it probably functions in polarized secretion.
