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Expression of beta-catenin is regulated by PI-3 kinase and sodium butyrate in colorectal cancer cells
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SYSNO ASEP 0024289 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Expression of beta-catenin is regulated by PI-3 kinase and sodium butyrate in colorectal cancer cells Title Exprese beta-kateninu je v kolorektálních nádorových buňkách regulována PI-3 kinázou a butyrátem sodným Author(s) Turečková, Jolana (UMG-J) RID
Kučerová, Dana (UMG-J)
Vojtěchová, Martina (UMG-J) RID
Šloncová, Eva (UMG-J)
Tuháčková, Zdena (UMG-J)Source Title International Journal of Molecular Medicine. - : Spandidos Publications - ISSN 1107-3756
Roč. 17, č. 1 (2006), s. 69-75Number of pages 7 s. Language eng - English Country GR - Greece Keywords PI-3 kinase ; sodium butyrate ; ribosomal protein S6 Subject RIV EB - Genetics ; Molecular Biology R&D Projects KJB5052302 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA301/04/0550 GA ČR - Czech Science Foundation (CSF) GP301/02/D159 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) Annotation β-catenin is implicated in intercellular junctions and transcriptional co-activation. Here we examined regulation of the expression of β-catenin in HT29 colorectal adenocarcinoma cells. Our results showed that inhibition of PI-3 kinase with wortmannin was accompanied by a considerably reduced expression of β-catenin. This effect was overcome by butyrate and occured at the protein level, not at the level of mRNA. Moreover, NaBT significantly increased phosphorylation of the ribosomal protein S6, participating in translational control of gene expression. This was accompanied by the increased phosphorylation of p70 S6K and MAPKs, the effector proteins that are upstream of protein S6 in the distinct signaling pathways. These facts indicate that different signaling pathways may be involved in the regulation of β-catenin synthesis. Modulation of β-catenin expression by NaBT occured at the level of protein translation, suggesting that NaBT may act as a translational regulator. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2006
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