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The Trypanosoma brucei La protein is a candidate poly(U) shield that impacts spliced leader RNA maturation and tRNA intron removal
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SYSNO ASEP 0023737 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title The Trypanosoma brucei La protein is a candidate poly(U) shield that impacts spliced leader RNA maturation and tRNA intron removal Title La protein Trypanosoma brucei je kandidátní poly(U) chránící protein, který ovlivňuje zrání spliced leader RNA a odstranění intronu z tRNA Author(s) Trantírková, Silvie (PAU-O) RID
Paris, Zdeněk (PAU-O)
Sturm, N. R. (US)
Campbell, D. A. (US)
Lukeš, Julius (PAU-O)Source Title International Journal for Parasitology. - : Elsevier - ISSN 0020-7519
Roč. 35, č. 4 (2005), s. 359-366Number of pages 8 s. Language eng - English Country AU - Australia Keywords splicing ; Trypanosoma ; RNA interference Subject RIV EB - Genetics ; Molecular Biology R&D Projects IAA5022302 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) Annotation By virtue of its preferential binding to poly(U) tails on small RNA precursors and nuclear localisation motif, the La protein has been implicated for a role in the stabilisation and nuclear retention of processing intermediates for a variety of small RNAs in eukaryotic cells. La protein was targeted for selective knockdown by inducible RNA interference in Trypanosoma brucei. In the relative absence of La protein T. brucei cells were not viable, in contrast to La gene knockouts in yeast. A variety of potential small RNA substrates were examined under induction, including spliced leader RNA, spliced leader associated RNA, the U1, U2, U4, and U6 small nuclear RNAs, 5S ribosomal RNA, U3 small nucleolar RNA, and tRNATyr. This study indicates that La protein binding is not essential for maturation of the spliced leader RNA, but does not rule out the presence of an alternative processing pathway that could compensate for the absence of normally-associated La protein. Workplace Institute of Parasitology (until 2005) Contact Kateřina Homrová,katerina.homrova@paru.cas.cz , Tel.: 387 775 406 Year of Publishing 2006
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