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Luminometric method for screening retroviral protease inhibitors
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SYSNO ASEP 0021541 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Luminometric method for screening retroviral protease inhibitors Title Luminometrická metoda pro testování inhibitorů retrovirové proteasy Author(s) Horáková, D. (CZ)
Rumlová, Michaela (UOCHB-X) RID
Pichová, Iva (UOCHB-X) RID, ORCID
Ruml, Tomáš (UOCHB-X)Source Title Analytical Biochemistry. - : Elsevier - ISSN 0003-2697
Roč. 345, č. 1 (2005), s. 96-101Number of pages 6 s. Language eng - English Country US - United States Keywords retroviral protease ; inhibitors ; luminescent assay Subject RIV CE - Biochemistry R&D Projects IAA4055304 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) 1M0508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40550506 - UOCHB-X (2005-2011) Annotation We have developed a sensitive luminometric assay for determining the activity of retroviral proteases that uses proteolytic cleavage of polypeptide substrate derived from the Gag precursor protein of Mason–Pfizer monkey virus (M-PMV) immobilized on Ni–NTA HisSorb Strips microplates. The cleavage of the substrate was monitored as a decrease in luminescent signal caused by the release of the cleavage product conjugated to HRP. Testing of a set of M-PMV protease inhibitors confirmed that this method is suitable for high-throughput screening of retroviral protease inhibitors. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2006
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