Alpha-Tocopheryl succinate induces cytostasis and apoptosis in osteosarcoma cells: the role of E2F1
1.
SYSNO ASEP
0001288
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Alpha-Tocopheryl succinate induces cytostasis and apoptosis in osteosarcoma cells: the role of E2F1
Title
Alfa-tokoferyl sukcinát vyvolává v buňkách osteosarkomu cytostázu a apoptózu: úloha E2F1
Author(s)
Alleva, R. (IT) Benassi, M.S. (IT) Tomasetti, M. (IT) Gellert, N. (AU) Ponticelli, F. (IT) Borghi, B. (IT) Picci, P. (IT) Neužil, Jiří (UMG-J)
Source Title
Biochemical and Biophysical Research Communications. - : Elsevier
- ISSN 0006-291X
Roč. 331, č. 4 (2005), s. 1515-1521
Number of pages
7 s.
Language
eng - English
Country
US - United States
Keywords
osteosarcoma ; alpha-tocopheryl succinate ; E2F1
Subject RIV
EB - Genetics ; Molecular Biology
CEZ
AV0Z50520514 - UMG-J (2005-2011)
AV0Z5052915 - UMG-J
Annotation
Alpha-tocopheryl succinate (alpha-TOS), a redox-inactive analog of vitamin E, induces cell cycle arrest, differentiation, and triggers apoptosis. We examined the ability of alpha-TOS to induce cytostasis and/or apoptosis in two human osteosarcoma cell lines, which carry wild-type pRb but differ in the p53 status. In the wt-p53 cells, alpha-TOS induced apoptosis, which was associated with p53 activation and enhanced E2F1 expression. Mutant p53 cells failed to undergo apoptosis when challenged with alpha-TOS. The cell growth arrest after alpha-TOS treatment was associated with a reduced expression of E2F1. Knocking down E2F1 rendered the alpha-TOS-sensitive cells rather resistant to the apoptotic stimulus inducing a marked and prolonged cell growth arrest. We conclude that alpha-TOS induces cell growth arrest or apoptosis involving E2F1.
