Preconcentration and Detection of Mercury with Bioluminescent Bioreporter E. coli ARL1.

0453525 - ÚCHP 2016 RIV DE eng J - Journal Article
Solovyev, Andrey - Koštejn, Martin - Kuncová, Gabriela - Dostálek, P. - Rohovec, Jan - Navrátil, Tomáš
Preconcentration and Detection of Mercury with Bioluminescent Bioreporter E. coli ARL1.
Applied Microbiology and Biotechnology. Roč. 99, č. 20 (2015), s. 8793-8802. ISSN 0175-7598. E-ISSN 1432-0614
Institutional support: RVO:67985858 ; RVO:67985831
Keywords : mercury detection * bioreporters * biosorbents
Subject RIV: CC - Organic Chemistry
Impact factor: 3.376, year: 2015

Cell wall envelopes treated with sodium hydroxide and spray-dried were used as mercury sorbents. The sorbent having sorption capacity 17.7 ± 0.1 μmol/g determined was employed for preconcentration of mercury containing 1–10 ng/L. After preconcentration, bioavailable mercury was detected in samples of soil, stream, and tap water via induction of bioluminescence of E. coli ARL1. Iron and manganese at concentrations of tenth microgram per liter interfered bioluminescence detection of mercury. In tap water was detected semiquantitatively 0.127 ± 0.1 nmol/L by the induction of bioluminescence of E. coli ARL1 in medium with tryptone after preconcentration using a method of standard addition.
Permanent Link: http://hdl.handle.net/11104/0254317