Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo

0350885 - ÚPT 2011 RIV CH eng J - Journal Article
Samek, Ota - Jonáš, Alexandr - Pilát, Zdeněk - Zemánek, Pavel - Nedbal, Ladislav - Tříska, Jan - Kotas, Petr - Trtílek, M.
Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo.
Sensors. Roč. 10, č. 9 (2010), s. 8635-8651. E-ISSN 1424-8220
R&D Projects: GA MŠMT ED0017/01/01; GA MŠMT OC08034; GA MPO FR-TI1/433; GA ČR GA206/09/1284
Grant - others:EU(XE) PERG 06-GA-2009-256526
Institutional research plan: CEZ:AV0Z20650511; CEZ:AV0Z60870520
Keywords : Raman spectroscopy * algal cells * lipids * iodine value
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 1.771, year: 2010

lgae are becoming a strategic source of fuels, food, feedstocks, and biologically active compounds. This potential has stimulated the development of innovative analytical methods focused on these microorganisms. Algal lipids are among the most promising potential products for fuels as well as for nutrition. The crucial parameter characterizing the algal lipids is the degree of unsaturation of the constituent fatty acids quantified by the iodine value. Here we demonstrate the capacity of the spatially resolved Raman microspectroscopy to determine the effective iodine value in lipid storage bodies of individual living algal cells. The Raman spectra were collected from three selected algal species immobilized in an agarose gel.
Permanent Link: http://hdl.handle.net/11104/0190765