Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies

0542103 - FGÚ 2022 RIV GB eng J - Journal Article
Ujčíková, Hana - Hejnová, L. - Eckhardt, Adam - Roubalová, Lenka - Novotný, J. - Svoboda, Petr
Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies.
Neurochemistry International. Roč. 144, Mar (2021), č. článku 104975. ISSN 0197-0186. E-ISSN 1872-9754
R&D Projects: GA ČR(CZ) GA19-03295S
Institutional support: RVO:67985823
Keywords : long-term morphine withdrawal * phosphoproteomic analysis * nLC-MS * label-free quantification * 14-3-3 proteins
OECD category: Physiology (including cytology)
Impact factor: 4.297, year: 2021
Method of publishing: Limited access
https://doi.org/10.1016/j.neuint.2021.104975

Opioid addiction is characterized by compulsive drug seeking and taking behavior, which is thought to result from persistent neuroadaptations. However, there is a lack of information about the changes at both the cellular and molecular levels occurring after cessation of drug administration. The aim of our study was to determine alterations of both phosphoproteome and proteome in selected brain regions of the rats (brain cortex, hippocampus, striatum, and cerebellum) 3 months after cessation of 10-day morphine treatment. Phosphoproteome profiling was performed by Pro-Q® Diamond staining. The gel-based proteomic approach accompanied by label-free quantification (MaxLFQ) was used for characterization of proteome changes.The phosphoproteomic analysis revealed the largest change in the hippocampus (14), only few altered proteins were detected in the forebrain cortex (5), striatum (4), and cerebellum (3). The change of total protein composition, determined by 2D electrophoresis followed by LFQ analysis, identified 22 proteins with significantly altered expression levels in the forebrain cortex, 19 proteins in the hippocampus, 12 in the striatum and 10 in the cerebellum. The majority of altered proteins were functionally related to energy metabolism and cytoskeleton reorganization. As the most important change we regard down-regulation of 14-3-3 proteins in rat cortex and hippocampus.Our findings indicate that i) different parts of the brain respond in a distinct manner to the protracted morphine withdrawal, ii) characterize changes of protein composition in these brain parts, and iii) enlarge the scope of evidence for adaptability and distinct neuroplasticity proceeding in the brain of drug-addicted organism.
Permanent Link: http://hdl.handle.net/11104/0319590