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Binding of Divalent Cations to Insulin: Capillary Electrophoresis and Molecular Simulations
- 1.0492074 - ÚFCH JH 2019 RIV US eng J - Journal Article
Dubué-Dijon, E. - Delcroix, Pauline - Martinez-Seara, H. - Hladílková, J. - Coufal, P. - Křížek, T. - Jungwirth, P.
Binding of Divalent Cations to Insulin: Capillary Electrophoresis and Molecular Simulations.
Journal of Physical Chemistry B. Roč. 122, č. 21 (2018), s. 5640-5648. ISSN 1520-6106. E-ISSN 1520-5207
R&D Projects: GA ČR(CZ) GA16-01074S
Institutional support: RVO:61388955
Keywords : neutron scattering * secretory granules * aqueous solutions
OECD category: Physical chemistry
Impact factor: 2.923, year: 2018
Method of publishing: Open access
In the present study, we characterize the binding of divalent cations to insulin in aqueous salt solutions by means of capillary electrophoresis and molecular dynamics simulations. The results show a strong pH dependence. At low pH, at which all the carboxylate groups are protonated and the protein has an overall positive charge, all the cations exhibit only weak and rather unspecific interactions with insulin. In contrast, at close to neutral pH, when all the carboxylate groups are deprotonated and negatively charged, the charge-neutralizing effect of magnesium, calcium, and zinc, in particular, on the electrophoretic mobility of insulin is significant. This is also reflected in the results of molecular dynamics simulations showing accumulation of cations at the protein surface, which becomes smaller in magnitude upon effective inclusion of electronic polarization via charge rescaling.
Permanent Link: http://hdl.handle.net/11104/0285641
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