Analysis of tissue-bound cells: Novel approaches using laser capture microdissection

0422682 - ÚIACH 2014 RIV CZ eng C - Conference Paper (international conference)
Matalová, Eva - Adamová, Eva - Klepárník, Karel
Analysis of tissue-bound cells: Novel approaches using laser capture microdissection.
Chemické Listy. Roč. 107, Issue s3.. Praha: Česká společnost chemická, 2013, s. 320-321. ISSN 0009-2770. E-ISSN 1213-7103.
[CECE Junior 2013. Brno (CZ), 12.11.2013-13.11.2013]
R&D Projects: GA MŠMT(CZ) EE2.3.20.0182; GA ČR GAP206/11/2377; GA ČR GCP302/12/J059
Institutional support: RVO:68081715 ; RVO:67985904
Keywords : apoptosis * laser capture microdissection * cell analysis
Subject RIV: CB - Analytical Chemistry, Separation

Recent biomedical research prefers analysis of homogenous samples obtained from primary cells to cell lines. Moreover, attention is paid to cells of low number but high biological impact, such as signalling centres in embryonic development, stem cells or tumour cells. To localize cells of interest within an organ/tissue, histological sections are widely used. However, the methods of choice for further analysis of such samples are mostly limited to histochemistry, immunohistochemistry and in situ hybridization.
Permanent Link: http://hdl.handle.net/11104/0228839