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Efficient disruption of endogenous Bombyx gene by TAL effector nucleases
- 1.0390514 - BC 2013 RIV GB eng J - Journal Article
Sajwan, Suresh - Takasu, Y. - Tamura, T. - Uchino, K. - Sezutsu, H. - Žurovec, Michal
Efficient disruption of endogenous Bombyx gene by TAL effector nucleases.
Insect Biochemistry and Molecular Biology. Roč. 43, č. 1 (2013), s. 17-23. ISSN 0965-1748. E-ISSN 1879-0240
R&D Projects: GA ČR GAP305/10/2406
Grant - others:Japan Society for the Promotion of Science(JP) 23580083
Institutional support: RVO:60077344
Keywords : gene targeting * ZFN * nonhomologous end joining
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.420, year: 2013
http://www.sciencedirect.com/science/article/pii/S0965174812001622#
TALENS are engineered nucleases used for targeted gene disruptions in various organisms. We examined the potential of using full length TALENs for site-specific mutagenesis in silkworm. Three full-length TALEN pairs were synthesized by Golden gate assembly, used for activity examination in heterologous yeast system and finally for mutagenesis in Bombyx mori germline. All TALENs were able to generate somatic and germline mutations in silkworms albeit at low frequency.
Permanent Link: http://hdl.handle.net/11104/0219546
Number of the records: 1