The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions

0364917 - BC 2012 RIV GB eng J - Journal Article
Hansel, R. - Loehr, F. - Foldynová-Trantírková, Silvie - Bamberg, E. - Trantírek, L. - Doetsch, V.
The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions.
Nucleic Acids Research. Roč. 39, č. 13 (2011), 5768-5775. ISSN 0305-1048. E-ISSN 1362-4962
Institutional research plan: CEZ:AV0Z60220518
Keywords : MOLECULAR CROWDING CONDITIONS * INTRAMOLECULAR G-QUADRUPLEX * CELL NMR-SPECTROSCOPY * C-MYC PROMOTER * K+ SOLUTION * DNA * STABILITY * PROTEIN * POLYMORPHISM * HYDRATION
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 8.026, year: 2011

G-quadruplex topologies of telomeric repeat sequences from vertebrates were investigated in the presence of molecular crowding (MC) mimetics, namely polyethylene glycol 200 (PEG), Ficoll 70 as well as Xenopus laevis egg extract by CD and NMR spectroscopy and native PAGE. Here, we show that the conformational behavior of the telomeric repeats in X. laevis egg extract or in Ficoll is notably different from that observed in the presence of PEG. While the behavior of the telomeric repeat in X. laevis egg extract or in Ficoll resembles results obtained under dilute conditions, PEG promotes the formation of high-order parallel topologies. Our data suggest that PEG should not be used as a MC mimetic.
Permanent Link: http://hdl.handle.net/11104/0200279