Occurrence of bifidobacteria in human milk

0359528 - ÚŽFG 2012 RIV DE eng J - Journal Article
Rada, V. - Nevoral, J. - Flajšmanová, K. - Ročková, Š. - Krčmová, I. - Grmanová, M. - Vlková, E. - Nováková, I. - Killer, Jiří - Kopečný, Jan
Occurrence of bifidobacteria in human milk.
Milchwissenschaft-Milk Science International. Roč. 66, č. 2 (2011), s. 123-126. ISSN 0026-3788
Grant - others:Ministerstvo zdravotnictví ČR(CZ) NR8310
Institutional research plan: CEZ:AV0Z50450515
Keywords : Human milk
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.350, year: 2011

Two groups of human milk samples were tested: The first group (18) originated from mothers with babies without bifidobacteria in their faecal flora (BIF-), the second (43) from mothers with babies with bifidobacteria in their faecal flora (BIF+). The occurrence of bifidobacteria in faeces was tested using cultivation method and FISH. For the detection of bifidobacteria in milk, the samples were subcultivated in TPY broth with mupirocin and subsequently the fructose-6-phosphate phosphoketolase activity (F6PPK) was determined. Infants of mothers from BIF+ group had high numbers of bifidobacteria (usually higher than 9 log CFU/g) in their faeces. On the contrary, infants of mothers from BIF- group did not contain detectable amounts of bifidobacteria in their faecal samples. These babies possessed significant amounts of clostridia in their faecal flora.
Permanent Link: http://hdl.handle.net/11104/0197304