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LC-ESI-MS/MS method for oxidative stress multimarker screening in the exhaled breath condensate of asbestosis/silicosis patients
- 1.0357968 - MBÚ 2011 RIV GB eng J - Journal Article
Syslová, K. - Kačer, P. - Kuzma, Marek - Pankrácová, A. - Fenclová, Z. - Vlčková, Š. - Lebedová, J. - Pelclová, D.
LC-ESI-MS/MS method for oxidative stress multimarker screening in the exhaled breath condensate of asbestosis/silicosis patients.
Journal of Breath Research. Roč. 4, č. 1 (2010), s. 1-8. ISSN 1752-7155. E-ISSN 1752-7163
R&D Projects: GA MZd NR9338
Institutional research plan: CEZ:AV0Z50200510
Keywords : 8-ISOPROSTANE * ASBESTOS * MARKERS
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.828, year: 2010
The sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers: 8-iso-prostaglandin F2α a lipid oxidation biomarker, o-tyrosine an amino acid oxidation biomarker and 8-hydroxy-2_-deoxy-guanosine a nucleic acid oxidation biomarker. The method consisted of a pre-treatment part, freeze drtiny (lyophilization), serving the purpose of biomarkers concentration from the exhaled breath condensate and detection method LC-ESI-MS/MS, where the selected reaction-monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification
Permanent Link: http://hdl.handle.net/11104/0196128
Number of the records: 1