Toward the crystallization of photosystem II core complex from Pisum sativum L

0355837 - ÚMG 2011 RIV US eng J - Journal Article
Prudnikova, T. - Gavira, J. A. - Řezáčová, Pavlína - Molina, E.P. - Hunalová, Ivana - Sviridova, E. - Schmidt, V. - Kohoutová, J. - Kutý, Michal - Kaftan, D. - Vácha, F. - Garcia-Ruiz, J. M. - Kutá-Smatanová, Ivana
Toward the crystallization of photosystem II core complex from Pisum sativum L.
Crystal Growth & Design. Roč. 10, č. 8 (2010), s. 3391-3396. ISSN 1528-7483. E-ISSN 1528-7505
R&D Projects: GA MŠMT(CZ) LC06010
Institutional research plan: CEZ:AV0Z50520514; CEZ:AV0Z50510513; CEZ:AV0Z60870520; CEZ:AV0Z40550506
Keywords : crystal-structure * cyanobacterial * elongatus
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.390, year: 2010

The crystallization of the higher plant similar to 1 gigadalton homodimeric lipid-pigment-protein complex of photosystem II constitutes a challenging undertaking that has not been crowned with success to date. In the continuation of our previous efforts (Kuta-Smatanova et al. Photosyn. Res. 2006, 90, 255-259) that had provided needle and plate crystals of plant photosystem II for the first time, we report on a study to crystallize three-dimensional crystals of the photosystem 11 from Pisum sativum L. suitable for X-ray analysis. In our screening, we optimized temperature, supersaturation rate, and concentrations of detergents, buffers, salts, and organic additives. We used a sitting and hanging drop vapor-diffusion method, microbatch under oil and counter-diffusion techniques, and direct and cross-influenced procedures. We have identified that the major limiting factors for the growth of crystals are the photosystem II stability and requirement of indispensable divalent cations.
Permanent Link: http://hdl.handle.net/11104/0006317