Engineered Delivery of Dental Stem-Cell-Derived Extracellular Vesicles for Periodontal Tissue Regeneration

0558806 - FGÚ 2023 RIV GB eng J - Journal Article
Zárubová, Jana - Hasani-Sadrabadi, M. M. - Dashtimoghadam, E. - Zhang, X. - Ansari, S. - Li, S. - Moshaverinia, A.
Engineered Delivery of Dental Stem-Cell-Derived Extracellular Vesicles for Periodontal Tissue Regeneration.
Advanced Healthcare Materials. Roč. 11, č. 12 (2022), č. článku 2102593. ISSN 2192-2640. E-ISSN 2192-2659
Institutional support: RVO:67985823
Keywords : extracellular vesicles * immunoengineering * local drug delivery * periodontal tissue healing * periodontitis
OECD category: Dentistry, oral surgery and medicine
Impact factor: 10, year: 2022
Method of publishing: Limited access
https://doi.org/10.1002/adhm.202102593

Periodontal disease begins as an inflammatory response to a bacterial biofilm deposited around the teeth, which over time leads to the destruction of tooth-supporting structures and consequently tooth loss. Conventional treatment strategies show limited efficacy in promoting regeneration of damaged periodontal tissues. Here, a delivery platform is developed for small extracellular vesicles (sEVs) derived from gingival mesenchymal stem cells (GMSCs) to treat periodontitis. EVs can achieve comparable therapeutic effects to their cells of origin. However, the short half-lives of EVs after their administration along with their rapid diffusion away from the delivery site necessitate frequent administration to achieve therapeutic benefits. To address these issues, “dual delivery” microparticles are engineered enabling microenvironment-sensitive release of EVs by metalloproteinases at the affected site along with antibiotics to suppress bacterial biofilm growth. GMSC sEVs are able to decrease the secretion of pro-inflammatory cytokines by monocytes/macrophages and T cells, suppress T-cell activation, and induce the formation of T regulatory cells (Tregs) in vitro and in a rat model of periodontal disease. One-time administration of immunomodulatory GMSC sEV-decorated microparticles leads to a significant improvement in regeneration of the damaged periodontal tissue. This approach will have potential clinical applications in the regeneration of a variety of tissues.
Permanent Link: https://hdl.handle.net/11104/0332573