Insight into the Mechanism of Action and Peptide‐Membrane Interactions of Aib‐Rich Peptides: Multitechnique Experimental and Theoretical Analysis

0542286 - ÚOCHB 2022 RIV DE eng J - Journal Article
Lizio, M. G. - Campana, M. - De Poli, M. - Jefferies, D. F. - Cullen, W. - Andrushchenko, Valery - Chmel, N. P. - Bouř, Petr - Khalid, S. - Clayden, J. - Blanch, E. - Rodger, A. - Webb, S. J.
Insight into the Mechanism of Action and Peptide‐Membrane Interactions of Aib‐Rich Peptides: Multitechnique Experimental and Theoretical Analysis.
Chembiochem. Roč. 22, č. 9 (2021), s. 1656-1667. ISSN 1439-4227. E-ISSN 1439-7633
R&D Projects: GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963
Keywords : antimicrobial peptides * lipid-peptide interactions * membranes * molecular dynamics * peptaibols * transfer of chirality
OECD category: Biophysics
Impact factor: 3.468, year: 2021
Method of publishing: Open access
https://doi.org/10.1002/cbic.202000834

The increase in resistant bacterial strains necessitates the identification of new antimicrobial molecules. Antimicrobial peptides (AMPs) are an attractive option because of evidence that bacteria cannot easily develop resistance to AMPs. The peptaibols, a class of naturally occurring AMPs, have shown particular promise as antimicrobial drugs, but their development has been hindered by their mechanism of action not being clearly understood. To explore how peptaibols might interact with membranes, circular dichroism, vibrational circular dichroism, linear dichroism, Raman spectroscopy, Raman optical activity, neutron reflectivity and molecular dynamics simulations have been used to study a small library of peptaibol mimics, the Aib‐rich peptides. All the peptides studied quickly partitioned and oriented in membranes, and we found evidence of chiral interactions between the phospholipids and membrane‐embedded peptides. The protocols presented in this paper open new ground by showing how chiro‐optical spectroscopies can throw light on the mechanism of action of AMPs.
Permanent Link: http://hdl.handle.net/11104/0319731