Protein interactions with quaternized chitosan/heparin multilayers

0463595 - ÚMCH 2017 RIV CZ eng J - Journal Article
Kumorek, Marta M. - Kubies, Dana - Riedel, Tomáš
Protein interactions with quaternized chitosan/heparin multilayers.
Physiological Research. Roč. 65, Suppl. 2 (2016), S253-S261. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA MŠMT(CZ) LQ1604
Institutional support: RVO:61389013
Keywords : heparin * chitosan * protein
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.461, year: 2016
http://www.biomed.cas.cz/physiolres/pdf/65%20Suppl%202/65_S253.pdf

Understanding the behavior of single proteins at the polyelectrolyte multilayer film/solution interface is of prime importance for the designing of bio-functionalized surface coatings. In the present paper, we study the adsorption of the model proteins, albumin and lysozyme, as well as basic fibroblast growth factor (FGF-2) on a polysaccharide multilayer film composed of quaternized chitosan and heparin. Several analytical methods were used to describe the formation of the polysaccharide film and its interactions with the proteins. Both albumin and lysozyme adsorbed on quaternized chitosan/heparin films, however this process strongly depended on the terminating polysaccharide. Protein adsorption was driven mainly by electrostatic interactions between protein and the terminal layer of the film. The effective binding of FGF-2 by the heparinterminated film suggested that other interactions could also contribute to the adsorption process. We believe that this FGF-2- presenting polysaccharide film may serve as a biofunctional surface coating for biologically-related applications.
Permanent Link: http://hdl.handle.net/11104/0264462