FAITH - Fast Assembly Inhibitor Test for HIV

0454191 - ÚOCHB 2016 RIV US eng J - Journal Article
Hadravová, Romana - Rumlová, Michaela - Ruml, T.
FAITH - Fast Assembly Inhibitor Test for HIV.
Virology. Roč. 486, Dec (2015), s. 78-87. ISSN 0042-6822. E-ISSN 1089-862X
R&D Projects: GA ČR(CZ) GA14-15326S; GA MŠMT LO1302; GA MŠMT(CZ) LO1304
Institutional support: RVO:61388963
Keywords : retrovirus * HIV * assembly * assay * inhibitor
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.200, year: 2015
http://www.sciencedirect.com/science/article/pii/S0042682215003864

Due to the high number of drug-resistant HIV-1 mutants generated by highly active antiretroviral therapy (HAART), there is continuing demand for new types of inhibitors. Both the assembly of the Gag polyprotein into immature and mature HIV-1 particles are attractive candidates for the blocking of the retroviral life cycle. Currently, no therapeutically-used assembly inhibitor is available. One possible explanation is the lack of a reliable and simple assembly inhibitor screening method. To identify compounds potentially inhibiting the formation of both types of HIV-1 particles, we developed a new fluorescent high-throughput screening assay. This assay is based on the quantification of the assembly efficiency in vitro in a 96-well plate format. The key components of the assay are HIV-1 Gag-derived proteins and a dual-labelled oligonucleotide, which emits fluorescence only when the assembly of retroviral particles is inhibited. The method was validated using three (CAI, BM2, FF74) reported assembly inhibitors.
Permanent Link: http://hdl.handle.net/11104/0254887