Pre-sorting endosomal transport of the GPI-anchored protein, CD59, is regulated by EHD1

0358085 - ÚMG 2011 RIV DK eng J - Journal Article
Cai, B. - Katafiasz, D. - Hořejší, Václav - Naslavsky, N.
Pre-sorting endosomal transport of the GPI-anchored protein, CD59, is regulated by EHD1.
Traffic. Roč. 12, č. 1 (2011), s. 102-120. ISSN 1398-9219. E-ISSN 1600-0854
Institutional research plan: CEZ:AV0Z50520514
Keywords : canine kidney cells * recycling compartment * receptor
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.919, year: 2011

The potential role of EHD1 in regulating the family of glycosylphosphatidylinositol-anchored proteins (GPI-APs) has not been determined. Here we demonstrate a novel role for EHD1 in regulating the trafficking of CD59, an endogenous GPI-AP, at early stages of trafficking through the endocytic pathway. EHD1 displays significant colocalization with newly internalized CD59. Upon EHD1 depletion, there is a rapid Rab5-independent coalescence of CD59 in the ERC region. However, expression of an active Arf6 mutant (Q67L), which traps internalized pre-sorting endosomal cargo in phosphatidylinositol(4,5)-bisphosphate enriched vacuoles, prevents this coalescence. It is of interest that sustained PKC activation leads to a similar coalescence of CD59 at the ERC, and treatment of EHD1-depleted cells with a PKC inhibitor (Go6976) blocked this rapid relocation of CD59. However, unlike sustained PKC activation, EHD1 depletion does not induce the translocation of PKC alpha to ERC.
Permanent Link: http://hdl.handle.net/11104/0196212