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Hyphenated ultra high-performance liquid chromatography–Nano Quantity
- 1.0327080 - MBÚ 2010 RIV NL eng J - Journal Article
Olšovská, Jana - Kameník, Zdeněk - Cajthaml, Tomáš
Hyphenated ultra high-performance liquid chromatography–Nano Quantity.
Journal of Chromatography A. Roč. 1216, č. 30 (2009), s. 5774-5778. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA MŠMT 1M06011
Institutional research plan: CEZ:AV0Z50200510
Keywords : macrolides * uv detection * ultra high-perfonmance liquid chromatography
Subject RIV: EE - Microbiology, Virology
Impact factor: 4.101, year: 2009
A novel universal aerosol-based detector Nano Quantity Analyte Detector – NQADTM, connected with an ultra-performance liquid chromatography system is described. The detector was employed for detection of selected antibiotic compounds – macrolides (oleandomycin, erythromycin, troleandomycin, clarithromycin and roxithromycin) that are hard to detect using classical UV detectors due to the lack of chromophores. The determined lowest detection limits under isocratic conditions for these compounds ranged from 3.0 to 5.4 μg/mL. The suitability of the detector connected with ultra high-performance liquid chromatography in the gradient mode was tested on a more complex mixture containing 12 antibiotics. The detector exhibited full compatibility under both the elution modes when UHPLC separations were achieved in relatively short run times
Permanent Link: http://hdl.handle.net/11104/0173980
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