Confocal and two-photon microscopy: foundations, applications, and advances

0142299 - FGU-C 20020021 RIV US eng M - Monography Chapter
Kubínová, Lucie - Janáček, Jiří - Krekule, Ivan
Stereological methods for estimating geometrical parameters of microscopic structure by three-dimensional imaging.
Confocal and two-photon microscopy: foundations, applications, and advances. Vol. 15. New York: WileyLiss, 2002 - (Diaspro, A.), s. 299-332. ISBN 0-471-40920-0
R&D Projects: GA AV ČR IAA5011810; GA ČR GA304/01/0257; GA ČR GA304/99/0680; GA MŠMT ME 184
Institutional research plan: CEZ:AV0Z5011922
Keywords : three-dimensional imagine * confocal microscopy
Subject RIV: EA - Cell Biology

Methods for estimating number, length, surface area and volume of microscopical structures, based on evaluation of three-dimensional image data obtained by confocal microscopy, are presented: (i) interactive unbiased stereological methods using virtual spatial grids generated by a computer applied to thick slices of arbitrary orientation; (ii) automatic digital methods based on image processing; (iii) automatic digital methods based on surface triangulation. The application of all methods, using our programs and software modules, is shown on practical examples. The methods are compared from the point of view of their applicability, efficiency and precision.
Permanent Link: http://hdl.handle.net/11104/0040005