The serine protease inhibitors and SERPINE1/2 disrupt prostaglandin E2 production and hyaluronic acid retention in FSH-stimulated pig cumulus-oocyte complexes

Blaha, Milan; Nevoral, J.; Procházka, Radek

doi:https://dx.doi.org/10.1111/rda.13497

Number of the records: 1

The serine protease inhibitors and SERPINE1/2 disrupt prostaglandin E2 production and hyaluronic acid retention in FSH-stimulated pig cumulus-oocyte complexes

1.

SYSNO ASEP	0517323
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The serine protease inhibitors and SERPINE1/2 disrupt prostaglandin E2 production and hyaluronic acid retention in FSH-stimulated pig cumulus-oocyte complexes
Author(s)	Blaha, Milan (UZFG-Y)_RID Nevoral, J. (CZ) Procházka, Radek (UZFG-Y)_{RID, ORCID}
Source Title	Reproduction in Domestical Animals. - : Wiley - ISSN 0936-6768 Roč. 54, Supplement 4 (2019), s. 65-68
Number of pages	4 s.
Publication form	Print - P
Language	eng - English
Country	US - United States
Keywords	cumulus cells ; follicle stimulating hormone ; plasmin
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Developmental biology
R&D Projects	QJ1510138 GA MZe - Ministry of Agriculture (MZe)
	EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UZFG-Y - RVO:67985904
UT WOS	000491448500011
EID SCOPUS	85073518025
DOI	10.1111/rda.13497
Annotation	Contents The serine proteases, tissue- and urokinase-type plasminogen activators (PLAT and PLAU) and their inhibitors SERPINE1/2 are regulators of plasminogen to plasmin conversion. They are widely expressed in ovarian tissues, including granulosa and cumulus cells, and their expression is regulated by gonadotropins. The aim of this work was to assess the effect of serine protease inhibitors (aprotinin and AEBSF) and SERPINE1/2 on FSH-induced cumulus cell expansion, the production of prostaglandin E2 (PGE2) and retention of hyaluronic acid (HA) in expanding cumulus. The serine protease activity proved to be essential for the production of PGE2 and also for the retention of HA, the inhibition of plasminogen activators by SERPINE1/2 had the same effect. Collectively, these data indicate that plasmin is required for proper function of expanding cumulus cells in vitro and presumably also in vivo in the pre-ovulatory follicles.
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2020
Electronic address	https://onlinelibrary.wiley.com/doi/full/10.1111/rda.13497

Number of the records: 1