Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation

0510268 - ÚŽFG 2020 RIV NL eng J - Journal Article
Fedorová, V. - Váňová, T. - Elrefae, L. - Pospíšil, J. - Petrášová, M. - Kolajová, V. - Hudačová, Z. - Baniariová, J. - Barak, M. - Pešková, L. - Bárta, T. - Kaucká, M. - Killinger, Michael - Večeřa, J. - Bernatík, O. - Čajánek, L. - Hříbková, H. - Bohaciaková, D.
Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation.
Stem Cell Research. Roč. 40, OCT (2019), č. článku 101563. ISSN 1873-5061. E-ISSN 1876-7753
Institutional support: RVO:67985904
Keywords : human embryonic stem cells * induced pluripotent stem cells * differentiation
OECD category: Developmental biology
Impact factor: 4.495, year: 2019
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S187350611930193X

Development of neural tube has been extensively modeled in vitro using human pluripotent stem cells (hPSCs) that are able to form radially organized cellular structures called neural rosettes. While a great amount of research has been done using neural rosettes, studies have only inadequately addressed how rosettes are formed and what the molecular mechanisms and pathways involved in their formation are. Here we address this question by detailed analysis of the expression of pluripotency and differentiation-associated proteins during the early onset of differentiation of hPSCs towards neural rosettes. Additionally, we show that the BMP signaling is likely contributing to the formation of the complex cluster of neural rosettes and its inhibition leads to the altered expression of PAX6, SOX2 and SOX1 proteins and the rosette morphology. Finally, we provide evidence that the mechanism of neural rosettes formation in vitro is reminiscent of the process of secondary neurulation rather than that of primary neurulation in vivo. Since secondary neurulation is a largely unexplored process, its understanding will ultimately assist the development of methods to prevent caudal neural tube defects in humans.
Permanent Link: http://hdl.handle.net/11104/0300781