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The synergistic effect of Selenium (selenite, -SeO32-) dose and irradiance intensity in Chlorella cultures
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SYSNO ASEP 0507324 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The synergistic effect of Selenium (selenite, -SeO32-) dose and irradiance intensity in Chlorella cultures Author(s) Babaei, Azadeh (MBU-M) ORCID
Ranglová, Karolína (MBU-M) ORCID
Malapascua, José R. (MBU-M)
Masojídek, Jiří (MBU-M) RID, ORCIDArticle number 56 Source Title AMB Express. - : Springer - ISSN 2191-0855
Roč. 7, MAR 7 (2017)Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords Chlorella ; Chlorophyll fluorescence ; Irradiance intensity Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000395980800001 EID SCOPUS 85014780026 DOI 10.1186/s13568-017-0348-7 Annotation Microalgae are able to metabolize inorganic selenium (Se) to organic forms (e.g. Se-proteins) nevertheless at certain Se concentration culture growth is inhibited. The aim of this work was to confirm the hypothesis that the limit of Se tolerance in Chlorella cultures is related to photosynthetic performance, i.e. depends on light intensity. We studied the relation between the dose and irradiance to find the range of Se tolerance in laboratory and outdoor cultures. At low irradiance (250 mu mol photons m(-2) s(-1)), the daily dose of Se below 8.5 mg per g of biomass (< 20 mu M) partially stimulated the photosynthetic activity (relative electron transport rate) and growth of Chlorella cultures (biomass density of similar to 1.5 g DW L-1) compared to the control (no Se added). It was accompanied by substantial Se incorporation to microalgae biomass (similar to 0.5 mg Se g(-1) DW). When the Se daily dose and level of irradiance were doubled (16 mg Se g(-1) DW 500 mu mol photons m(-2) s(-1)), the photosynthetic activity and growth were stimulated for several days and ample incorporation of Se to biomass (7.1 mg g(-1) DW) was observed. Yet, the same Se daily dose under increased irradiance (750 mu mol photons m(-2) s(-1)) caused the synergistic effect manifested by significant inhibition of photosynthesis, growth and lowered Se incorporation to biomass. In the present experiments Chl fluorescence techniques were used to monitor photosynthetic activity for determination of optimal Se doses in order to achieve efficient incorporation without substantial inhibition of microalgae growth when producing Se-enriched biomass. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2020 Electronic address https://amb-express.springeropen.com/articles/10.1186/s13568-017-0348-7
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