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Enucleolation and nucleolus transfer in mammalian oocytes and zygotes
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SYSNO ASEP 0504368 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Enucleolation and nucleolus transfer in mammalian oocytes and zygotes Author(s) Benc, M. (SK)
Fulka Jr., J. (CZ)
Strejček, F. (SK)
Morovič, M. (SK)
Murín, Matěj (UZFG-Y) ORCID
Martínková, S. (CZ)
Jettmarová, D. (CZ)
Laurinčík, Jozef (UZFG-Y) ORCIDSource Title International Journal of Developmental Biology. - : Universidad del Pais Vasco - ISSN 0214-6282
Roč. 63, 3-5 (2019), s. 253-258Number of pages 6 s. Publication form Print - P Language eng - English Country ES - Spain Keywords nucleous ; micromanipulation ; oocyte Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology R&D Projects EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 000464941000018 DOI 10.1387/ijdb.190002mb Annotation The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygot PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easily manipulated, i.e. removed from GVs/PNs or eventually transferred into another oocyte/zygote. Thus, with the help of micromanipulation techniques it was possible to uncover the real function(s) they play in processes of oocyte maturation and early embryonic development. The purpose of our review is to describe briefly the micromanipulation techniques that can be used for oocyte/zygote nucleoli manipulation. Moreover, we present some examples of results that were obtained in nucleolus manipulation experiments. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2020 Electronic address http://www.ijdb.ehu.es/web/paper/190002mb/enucleolation-and-nucleolus-transfer-in-mammalian-oocytes-and-zygotes
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