Arginine-rich cell-penetrating peptides induce membrane multilamellarity and subsequently enter via formation of a fusion pore

Allolio, Christoph; Magarkar, Aniket; Jurkiewicz, Piotr; Baxová, Katarína; Javanainen, Matti; Mason, Philip E.; Šachl, Radek; Cebecauer, Marek; Hof, Martin; Horinek, D.; Heinz, V.; Rachel, R.; Ziegler, C. M.; Schröfel, A.; Jungwirth, Pavel

doi:https://dx.doi.org/10.1073/pnas.1811520115

Number of the records: 1

Arginine-rich cell-penetrating peptides induce membrane multilamellarity and subsequently enter via formation of a fusion pore

1.

SYSNO ASEP	0498461
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Arginine-rich cell-penetrating peptides induce membrane multilamellarity and subsequently enter via formation of a fusion pore
Author(s)	Allolio, Christoph (UOCHB-X)_ORCID Magarkar, Aniket (UOCHB-X) Jurkiewicz, Piotr (UFCH-W)_{RID, ORCID} Baxová, Katarína (UOCHB-X) Javanainen, Matti (UOCHB-X)_{RID, ORCID} Mason, Philip E. (UOCHB-X)_{RID, ORCID} Šachl, Radek (UFCH-W)_{RID, ORCID} Cebecauer, Marek (UFCH-W)_{RID, ORCID, SAI} Hof, Martin (UFCH-W)_{RID, ORCID} Horinek, D. (DE) Heinz, V. (DE) Rachel, R. (DE) Ziegler, C. M. (DE) Schröfel, A. (CZ) Jungwirth, Pavel (UOCHB-X)_{RID, ORCID}
Source Title	Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424 Roč. 115, č. 47 (2018), s. 11923-11928
Number of pages	6 s.
Language	eng - English
Country	US - United States
Keywords	cell-penetrating peptide ; membrane fusion ; fluorescence microscopy ; electron microscopy ; molecular dynamics
Subject RIV	CF - Physical ; Theoretical Chemistry
OECD category	Physical chemistry
Subject RIV - cooperation	J. Heyrovsky Institute of Physical Chemistry - Physical ; Theoretical Chemistry
R&D Projects	GA16-01074S GA ČR - Czech Science Foundation (CSF)
	GA17-03160S GA ČR - Czech Science Foundation (CSF)
Institutional support	UOCHB-X - RVO:61388963 ; UFCH-W - RVO:61388955
UT WOS	000450642800040
EID SCOPUS	85056778564
DOI	10.1073/pnas.1811520115
Annotation	Arginine-rich cell-penetrating peptides do not enter cells by directly passing through a lipid membrane, they instead passively enter vesicles and live cells by inducing membrane multilamellarity and fusion. The molecular picture of this penetration mode, which differs qualitatively from the previously proposed direct mechanism, is provided by molecular dynamics simulations. The kinetics of vesicle agglomeration and fusion by an iconic cell-penetrating peptide-nonaarginine-are documented via real-time fluorescence techniques, while the induction of multilamellar phases in vesicles and live cells is demonstrated by a combination of electron and fluorescence microscopies. This concert of experiments and simulations reveals that the identified passive cell penetration mechanism bears analogy to vesicle fusion induced by calcium ions, indicating that the two processes may share a common mechanistic origin.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
Year of Publishing	2019
Electronic address	https://www.pnas.org/content/115/47/11923

Number of the records: 1