Freezing processes in cryoprotective solutions of ME.sub.2./sub.SO, trehalose and antifreeze protein APAFP752 and their impact on chromatin condition of cryopreserved cells

0494040 - FZÚ 2019 RIV US eng A - Abstract
Golan, Martin - Sedláková, Silvia - Mičová, Júlia - Šebera, Jakub - Kratochvílová, Irena - Richter, J. - Řeha, David - Falk, Martin - Falková, Iva - Bačíková, Alena - Pagáčová, Eva - Varga, K.
Freezing processes in cryoprotective solutions of ME₂SO, trehalose and antifreeze protein APAFP752 and their impact on chromatin condition of cryopreserved cells.
Cryobiology. Elsevier. Roč. 71, č. 3 (2015), s. 561-561. ISSN 0011-2240. E-ISSN 1090-2392
Institutional support: RVO:68378271 ; RVO:61388971 ; RVO:68081707
Keywords : cryoprotective agents * freezing * cryopreservation
OECD category: Biophysics

In our work, we studied the behaviour of aqueous solutions of cryoprotective agents (Me2SO, trehalose, antifreeze protein ApAFP752) upon freezing/thawing. We characterized the course of phase change of all the s ranging from 20°C to -170°C using differential scanning calorimetry (shape and position of freezing/thawing curves) and Raman spectroscopy (molecular and intermolecular structure). We correlate our experimental results with molecular dynamics‘ modelling of interactions of solution constituents. We also show that antifreeze protein ApAFP752 has thermal hysteresis activity and is able to increase viability of Xenopus oocytes when microinjected into them prior to cryopreservation.
Permanent Link: http://hdl.handle.net/11104/0287284