Number of the records: 1
Communication between N terminus and loop2 tunes Orai activation
- 1.
SYSNO ASEP 0489833 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Communication between N terminus and loop2 tunes Orai activation Author(s) Fahrner, M. (AT)
Pandey, Saurabh Kumar (MBU-M) ORCID
Muik, M. (AT)
Traxler, L. (AT)
Butorac, C. (AT)
Stadlbauer, M. (AT)
Zayats, Vasilina (MBU-M) ORCID
Křížová, A. (AT)
Plenk, P. (AT)
Frischauf, I. (AT)
Schindl, R. (AT)
Gruber, H.J. (AT)
Hinterdorfer, P. (AT)
Ettrich, Rüdiger (MBU-M)
Romanin, C. (AT)
Derler, I. (AT)Source Title Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 293, č. 4 (2018), s. 1271-1285Number of pages 15 s. Language eng - English Country US - United States Keywords atomic force microscopy (AFM) ; calcium release-activated calcium channel protein 1 (ORAI1) ; electrophysiology Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LTC17069 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000423515000014 EID SCOPUS 85041209005 DOI 10.1016/j.saa.2017.12.021 Annotation The CRAC channel gating involves the binding of STIM1 C-terminus to cytosolic N- and C-termini of Orai. The C-termini of Orai is the main binding partner however N-termini also have been found necessary for Orai channel function. Despite a highly conserved ETON region Orai1 and Orai3 differ in length of the ETON region. Our aim here was to study the reasons for different behavior of these two isoforms and to find the differences in structural requirement for gating and function. While there is no interaction between the N-terminus and loop2 in Orai3, we predict that interactions between loop2 and Orai1 N-terminus results into masking the STIM1 coupling sites which are located either at N-terminus or loop2 or both, thus loop2 either directly or allosterically affects the STIM1-Orai coupling in an isoform specific manner. Thus along with the requirement of conserved ETON region a fine tuning between N-termini and loop2 conformations has an essential role in maintaining permissive conformation of channel and store-operated function of Orai channel.
Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2019
Number of the records: 1