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Osteogenic Potential of Caspases Related to Endochondral Ossification
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SYSNO ASEP 0489550 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Osteogenic Potential of Caspases Related to Endochondral Ossification Author(s) Janečková, E. (CZ)
Bíliková, P. (CZ)
Matalová, Eva (UZFG-Y) RIDSource Title Journal of Histochemistry and Cytochemistry. - : Sage - ISSN 0022-1554
Roč. 66, č. 1 (2018), s. 47-58Number of pages 12 s. Publication form Print - P Language eng - English Country US - United States Keywords caspases ; endochronal ossification ; growth plate Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology Institutional support UZFG-Y - RVO:67985904 UT WOS 000418835500004 EID SCOPUS 85039734166 DOI 10.1369/0022155417739283 Annotation Caspases have functions particularly in apoptosis and inflammation. Increasing evidence indicates novel roles of these proteases in cell differentiation, including those involved in osteogenesis. This investigation provides a complex screening of osteogenic markers affected by pan caspase inhibition in micromass cultures derived from mouse forelimbs. PCR Array analysis showed significant alterations in expression of 49 osteogenic genes after 7 days of inhibition. The largest change was a decrease in CD36 expression, which was confirmed at organ level by caspase inhibition in cultured mouse ulnae followed by CD36 immunohistochemical analysis. So far, available data point to osteogenic potential of pro-apoptotic caspases. Therefore, the expression of pro-apoptotic caspases (-3, -6, -7, -8, -9) within the growth plate of mouse forelimbs at the stage where the individual zones are clearly apparent was studied. Caspase-9 was reported in the growth plate for the first time as well as caspase-6 and -7 in the resting zone, caspase-7 in the proliferation, and caspase-6 and -8 in the ossification zone. For all caspases, there was a gradient increase in activation toward the ossification zone. The distribution of staining varied significantly from that of apoptotic cells, and thus, the results further support non-apoptotic participation of caspases in osteogenesis. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2019
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