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Utilization of organic nitrogen by arbuscular mycorrhizal fungi-is there a specific role for protists and ammonia oxidizers?
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SYSNO ASEP 0489043 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Utilization of organic nitrogen by arbuscular mycorrhizal fungi-is there a specific role for protists and ammonia oxidizers? Author(s) Bukovská, Petra (MBU-M) RID, ORCID
Bonkowski, M. (DE)
Konvalinková, Tereza (MBU-M) RID, ORCID
Beskid, Olena (MBU-M) RID
Hujslová, Martina (MBU-M) RID, ORCID
Püschel, David (MBU-M) RID, ORCID
Řezáčová, Veronika (MBU-M) ORCID
Gutierrez-Nunez, M.S. (MX)
Gryndler, Milan (MBU-M) RID, ORCID
Jansa, Jan (MBU-M) RID, ORCIDSource Title Mycorrhiza. - : Springer - ISSN 0940-6360
Roč. 28, č. 3 (2018), s. 269-283Number of pages 15 s. Language eng - English Country US - United States Keywords N-15-labeling ; Metatranscriptomics ; Organic nitrogen (N) Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA18-04892S GA ČR - Czech Science Foundation (CSF) LK11224 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000427387100007 EID SCOPUS 85042117862 DOI 10.1007/s00572-018-0825-0 Annotation Arbuscular mycorrhizal (AM) fungi can significantly contribute to plant nitrogen (N) uptake from complex organic sources, most likely in concert with activity of soil saprotrophs and other microbes releasing and transforming the N bound in organic forms. Here, we tested whether AM fungus (Rhizophagus irregularis) extraradical hyphal networks showed any preferences towards certain forms of organic N (chitin of fungal or crustacean origin, DNA, clover biomass, or albumin) administered in spatially discrete patches, and how the presence of AM fungal hyphae affected other microbes. By direct N-15 labeling, we also quantified the flux of N to the plants (Andropogon gerardii) through the AM fungal hyphae from fungal chitin and from clover biomass. The AM fungal hyphae colonized patches supplemented with organic N sources significantly more than those receiving only mineral nutrients, organic carbon in form of cellulose, or nothing. Mycorrhizal plants grew 6.4-fold larger and accumulated, on average, 20.3-fold more N-15 originating from the labeled organic sources than their nonmycorrhizal counterparts. Whereas the abundance of microbes (bacteria, fungi, or Acanthamoeba sp.) in the different patches was primarily driven by patch quality, we noted a consistent suppression of the microbial abundances by the presence of AM fungal hyphae. This suppression was particularly strong for ammonia oxidizing bacteria. Our results indicate that AM fungi successfully competed with the other microbes for free ammonium ions and suggest an important role for the notoriously understudied soil protists to play in recycling organic N from soil to plants via AM fungal hyphae. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2019
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