Voltammetric Determination of Tumor Biomarkers using Flow Injection Analysis with Amperometric Detection
1.
SYSNO ASEP
0479846
Document Type
C - Proceedings Paper (int. conf.)
R&D Document Type
Conference Paper
Title
Voltammetric Determination of Tumor Biomarkers using Flow Injection Analysis with Amperometric Detection
Author(s)
Makrlíková, Anna (UFCH-W) Matysik, F.-M. (DE) Barek, J. (CZ) Vyskočil, V. (CZ)
Source Title
Proceedings of the 13th International Students Conference ”Modern Analytical Chemistry”. - Prague : Charles University in Prague, Faculty of Science, 2017 / Nesměrák K.
- ISBN 978-80-7444-052-6
Pages
s. 7-11
Number of pages
5 s.
Publication form
Print - P
Action
International Students Conference ”Modern Analytical Chemistry” /13./
Three tumor biomarkers (homovanillic acid, vanillylmandelic acid, and 5-hydroxyindole-3-acetic acid) have been determined by flow injection analysis with amperometric detection at screen-printed carbon electrodes in optimum medium of Britton-Robinson buffer (0.04 mol l-1, pH = 2.0). Dependences of the peaks current on the concentration of biomarkers were linear in the tested concentration region from 0.05 to 100 mu mol l-1, with the limits of detection of 0.065 mu mol l-1 for homovanillic acid, 0.053 mu mol l-1 for vanillylmandelic acid, and 0.033 mu mol l-1 for 5-hydroxyindole-3-acetic acid (calculated from heights), and 0.024 mu mol 1-1 for homovanillic acid, 0.020 mu mol l-1 forvanillylmandelic acid, and 0.012 mu mol l-1 for 5-hydroxyindole-3-acetic acid (calculated from areas), respectively.
