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Polymeric nanofibrous scaffolds reinforced with diamond and ceramic nanoparticles for bone tissue engineering
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SYSNO ASEP 0470313 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Polymeric nanofibrous scaffolds reinforced with diamond and ceramic nanoparticles for bone tissue engineering Author(s) Bačáková, Lucie (FGU-C) RID, ORCID
Pařízek, Martin (FGU-C) RID
Staňková, Ľubica (FGU-C) RID, ORCID
Novotná, Katarína (FGU-C)
Douglas, T.E.L. (BE)
Brady, M. A. (GB)
Kromka, Alexander (FZU-D) RID, ORCID, SAI
Potocký, Štěpán (FZU-D) RID, ORCID
Stránská, D. (CZ)Source Title NANOCON 2014. 6th International conference proceedings. - Ostrava : TANGER, 2015 - ISBN 978-80-87294-53-6 Pages s. 502-507 Number of pages 6 s. Publication form Print - P Action International Conference NANOCON /6./ Event date 05.11.2014-07.11.2014 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords material nanostructure ; nanofibers ; osteogenic cells ; bone implants ; regenerative medicine Subject RIV EI - Biotechnology ; Bionics R&D Projects GBP108/12/G108 GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 ; FZU-D - RVO:68378271 UT WOS 000350636300086 EID SCOPUS 85006074169 Annotation Three types of nanofibrous scaffolds were prepared by electrospining: (1) poly(lactide-co-glycoside) (PLGA) scaffolds reinforced with 23 wt.% of diamond nanoparticles (DNPs), (2) poly(L-lactide) (PLLA) scaffolds with DNPs in concentration ranging from from 0.4 wt.% to 12.3 wt.%, and (3) PLLA scaffolds with 5 wt.% or 15 wt.% of hydroxyapatite (HAp) nanoparticles. The diameter of the nanofibers ranged between 160 and 729 nm. The nanofibers with nanoparticles were thicker and the void spaces among them were smaller. Mechanical properties of the nanoparticle-loaded scaffolds were better, as demonstrated by a rupture test in scaffolds with DNPs and by a creep behavior test in scaffolds with HAp. On PLGA scaffolds with DNPs, the human osteoblast-like MG-63 cells adhered in similar numbers and grew with similar kinetics as on pure PLGA scaffolds. Human bone marrow mesenchymal stem cells grew faster and reached higher population densities on PLGA-DNP scaffolds. However, on PLLA-based scaffolds, the activity of mitochondrial enzymes and concentration of osteocalcin in MG-63 cells decreased with increasing DNP concentration. On the other hand, the metabolic activity of MG-63 cells and content of osteocalcin in these cells were positively correlated with the HAp concentration in PLLA scaffolds. Thus, PLGA nanofibers with 23 wt% of DNPs and PLLA nanofibers with 5 and particularly 15 wt.% of HAp seem to be promising for bone tissue engineering. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2017
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