Assessing HCH isomer uptake in Alnus glutinosa: implications for phytoremediation and microbial response

0587333 - ÚEB 2025 RIV US eng J - Journal Article
Amirbekov, A. - Vrchovecká, S. - Říha, J. - Petřík, Ivan - Friedecký, D. - Novák, Ondřej - Černík, M. - Hrabák, P. - Ševců, A.
Assessing HCH isomer uptake in Alnus glutinosa: implications for phytoremediation and microbial response.
Scientific Reports. Roč. 14, č. 1 (2024), č. článku 4187. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Keywords : haloalkane dehalogenase linb * gamma-hexachlorocyclohexane degradation * alpha-hexachlorocyclohexane * delta-hexachlorocyclohexane * beta-hexachlorocyclohexane * bacterial community * contaminated sites * gene-expression * soil * biodegradation
OECD category: Biochemistry and molecular biology
Impact factor: 4.6, year: 2022
Method of publishing: Open access
https://doi.org/10.1038/s41598-024-54235-1

Although the pesticide hexachlorocyclohexane (HCH) and its isomers have long been banned, their presence in the environment is still reported worldwide. In this study, we investigated the bioaccumulation potential of alpha, beta, and delta hexachlorocyclohexane (HCH) isomers in black alder saplings (Alnus glutinosa) to assess their environmental impact. Each isomer, at a concentration of 50 mg/kg, was individually mixed with soil, and triplicate setups, including a control without HCH, were monitored for three months with access to water. Gas chromatography-mass spectrometry revealed the highest concentrations of HCH isomers in roots, decreasing towards branches and leaves, with delta-HCH exhibiting the highest uptake (roots-14.7 mu g/g, trunk-7.2 mu g/g, branches-1.53 mu g/g, leaves-1.88 mu g/g). Interestingly, alpha-HCH was detected in high concentrations in beta-HCH polluted soil. Phytohormone analysis indicated altered cytokinin, jasmonate, abscisate, and gibberellin levels in A. glutinosa in response to HCH contamination. In addition, amplicon 16S rRNA sequencing was used to study the rhizosphere and soil microbial community. While rhizosphere microbial populations were generally similar in all HCH isomer samples, Pseudomonas spp. decreased across all HCH-amended samples, and Tomentella dominated in beta-HCH and control rhizosphere samples but was lowest in delta-HCH samples.
Permanent Link: https://hdl.handle.net/11104/0354568