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Short tRNA anticodon stem and mutant eRF1 allow stop codon reassignment (vol 613, pg 751, 2023)
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SYSNO ASEP 0583675 Document Type O - Others R&D Document Type The record was not marked in the RIV Title Short tRNA anticodon stem and mutant eRF1 allow stop codon reassignment (vol 613, pg 751, 2023) Author(s) Kachale, Ambar (BC-A) SAI, ORCID
Nenarokova, Anna (BC-A) RID, ORCID
Roithová, Adriana (MBU-M)
Durante, Ignacio Miguel (BC-A) RID, ORCID
Miletínová, Petra (MBU-M)
Záhonová, Kristýna (BC-A) ORCID
Nenarokov, Serafim (BC-A) RID, ORCID
Votýpka, Jan (BC-A) RID, ORCID
Horáková, Eva (BC-A) RID, ORCID
Ross, R.L. (US)
Yurchenko, V. (CZ)
Beznosková, Petra (MBU-M) RID, ORCID
Paris, Zdeněk (BC-A) RID, ORCID
Valášek, Leoš Shivaya (MBU-M) RID, ORCID
Lukeš, Julius (BC-A) RID, ORCIDNumber of authors 16 Year of issue 2024 Source Title Nature. - : Palgrave Macmillan - ISSN 0028-0836
Roč. 626, č. 7998 (2024), E4-E4Number of pages 1 s. Language eng - English Country GB - United Kingdom Keywords tRNA ; eRF1 ; codon Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology Subject RIV - cooperation Institute of Microbiology - Biochemistry Institutional support BC-A - RVO:60077344 ; MBU-M - RVO:61388971 UT WOS 001159144100030 DOI https://doi.org/10.1038/s41586-024-07065-0 Annotation After this article was published, we were alerted by Estienne Swart that the putative C. magnum 5-bp-long AS tRNATrp CCA, which we used as a negative control in Fig. 3d and 3f, most likely originated from a bacterial contaminant in the ciliate genome assembly. Using a metagenome binning strategy, we found that the contig containing the respective tRNA gene indeed belongs to a single bacterial metagenome-assembled genome (MAG) reconstructed from the whole-genome data. This high-quality MAG corresponds to an unidentified alphaproteobacterium not closely related to previously described taxa. We thus confirmed the bacterial origin of 5-bp AS tRNATrp. Regrettably, the presence of contaminating bacterial sequences in the C. magnum genome assembly was not obvious from the original paper1 when we were working on the original manuscript. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2025 Electronic address https://doi.org/10.1038/s41586-024-07065-0
Number of the records: 1